A human skull (Public Domain) and tobacco. (Public Domain)

Researchers Take a Bite Out of Prehistoric Nicotine Use

A team of scientists including researchers from Washington State University has shown for the first time that nicotine residue can be extracted from plaque, also known as "dental calculus," on the teeth of ancient tobacco users.

Their research provides a new method for determining who was consuming tobacco in the ancient world and could help trace the use of tobacco and other intoxicating plants further back into prehistory.

"The ability to identify nicotine and other plant-based drugs in ancient dental plaque could help us answer longstanding questions about the consumption of intoxicants by ancient humans," said Shannon Tushingham, a WSU assistant professor of anthropology and co-author of a new study on the research in Journal of Archaeological Science Reports. "For example, it could help us determine whether all members of society used tobacco, or only adults, or only males or females."

Tobacco plants

Tobacco plants. (Public Domain)

A new source of information

Tracing the ancient spread of tobacco in the Americas has traditionally relied on the presence of pipes, charred tobacco seeds and the analysis of hair and fecal matter. However, these items are rare in the archeological record and are hard to link to particular individuals. As a result, tobacco use has been difficult to document archeologically.

An examination in the 1970s of the mummy of Ramesses II revealed fragments of tobacco leaves in its abdomen. ( CC BY SA 3.0 )

An examination in the 1970s of the mummy of Ramesses II revealed fragments of tobacco leaves in its abdomen. ( CC BY SA 3.0 )

On the other hand, dental plaque adheres to the surfaces of teeth and mineralizes over time, preserving a wide range of substances that are in the mouth. It is easy to link to particular individuals because it can be removed directly from teeth.

Nevertheless, dental plaque was largely ignored by archaeologists until recently.

However, using modern and highly sensitive instrumentation, scientists have found they can detect and characterize trace amounts of a wide variety of compounds, including proteins, bacterial DNA, starch grains and other plant fibers in dental plaque.

Because nicotine is detectable in the dental plaque of contemporary smokers, Tushingham and her collaborators wanted to find out if it also preserved in plaque taken from people who lived long ago.

Example of ancient human teeth from the Qesem Cave, Israel.

Example of ancient human teeth from the Qesem Cave, Israel. (Prof. Israel Hershkovitz, Tel Aviv University/CC BY 4.0)

She and David Gang, a professor in the WSU Institute of Biological Chemistry, Korey Brownstein, a graduate student in the WSU Molecular Plant Sciences Program, and Jelmer Eerkens, an anthropologist at the University of California, Davis, collaborated with members from the Ohlone tribe in San Francisco Bay to extract plaque from the teeth of eight individuals, buried between 6,000 and 300 years ago, and analyze it for nicotine.

Using ordinary dental picks, Eerkens and his team at UC Davis extracted the dental plaque from the ancient teeth and then sent it to Tushingham and Gang's labs at WSU for analysis. The WSU researchers used liquid chromatography-mass spectrometry to test the samples for nicotine and other plant-based drugs like caffeine and atropine, a muscle-relaxant.

Among the samples they analyzed, two tested positive for nicotine, demonstrating for the first time that the drug can survive in detectable amounts in ancient plaque. One of these individuals, an adult man, was also buried with a pipe. A surprise came from the molar of an older woman, which also tested positive for nicotine.

Example of a clay tobacco pipe and a pipe case, possibly made of walnut. (Wellcome Images/CC BY 4.0)

Example of a clay tobacco pipe and a pipe case, possibly made of walnut. (Wellcome Images/CC BY 4.0)

"While we can't make any broad conclusions with this single case, her age, sex, and use of tobacco is intriguing," Eerkens said. "She was probably past child-bearing age, and likely a grandmother. This supports recent research suggesting that younger adult women in traditional societies avoid plant toxins like nicotine to protect infants from harmful biochemicals, but that older women can consume these intoxicants as needed or desired."

A woman smoking a pipe. (Roel Coutinho/CC BY SA 4.0)

A woman smoking a pipe. (Roel Coutinho/CC BY SA 4.0)

While the researchers did not detect evidence of any other plant-based drugs in this particular study, they believe dental plaque could be used help trace the use and spread of other intoxicants as well.

"We think a wide variety of plant-based, intoxicating chemicals could be detected in ancient dental plaque," Brownstein said. "It really opens up a lot of interesting avenues of discovery."

Top Image: A human skull (Public Domain) and tobacco. (Public Domain)

The article, originally titled ‘Nicotine extracted from ancient dental plaque for the first time’, was originally published on Science Daily.

Source: Washington State University. "Nicotine extracted from ancient dental plaque for the first time." ScienceDaily. ScienceDaily, 27 February 2018.

Register to become part of our active community, get updates, receive a monthly newsletter, and enjoy the benefits and rewards of our member point system OR just post your comment below as a Guest.

Human Origins

Silhouettes (Public Domain) in front of blood cells (Public Domain) and a gene.
Most people who have the Rh blood type are Rh-positive. There are also instances, however, where people are Rh-Negative. Health problems may occur for the unborn child of a mother with Rh-Negative blood when the baby is Rh-Positive.

Ancient Technology

Our Mission

At Ancient Origins, we believe that one of the most important fields of knowledge we can pursue as human beings is our beginnings. And while some people may seem content with the story as it stands, our view is that there exists countless mysteries, scientific anomalies and surprising artifacts that have yet to be discovered and explained.

The goal of Ancient Origins is to highlight recent archaeological discoveries, peer-reviewed academic research and evidence, as well as offering alternative viewpoints and explanations of science, archaeology, mythology, religion and history around the globe.

We’re the only Pop Archaeology site combining scientific research with out-of-the-box perspectives.

By bringing together top experts and authors, this archaeology website explores lost civilizations, examines sacred writings, tours ancient places, investigates ancient discoveries and questions mysterious happenings. Our open community is dedicated to digging into the origins of our species on planet earth, and question wherever the discoveries might take us. We seek to retell the story of our beginnings. 

Ancient Image Galleries

View from the Castle Gate (Burgtor). (Public Domain)
Door surrounded by roots of Tetrameles nudiflora in the Khmer temple of Ta Phrom, Angkor temple complex, located today in Cambodia. (CC BY-SA 3.0)
Cable car in the Xihai (West Sea) Grand Canyon (CC BY-SA 4.0)
Next article